Any beekeeper planning to permanently (sale) or temporarily (commercial pollination) import colonies, nuclei, packages of bees or queens into the territory of Québec must have an authorization to import bees into Québec issued by the Direction de la santé animale (DSA) of the Ministère de l’Agriculture, des Pêcheries et de l’Alimentation (MAPAQ). This protocol contributes to ensure a reasonable degree of protection against the risk of introducing Aethina tumida (small hive beetle or SHB) and Paenibacillus larvae (the causative organism of American foulbrood), bee diseases designated in the Regulation to designate contagious or parasitic diseases, infectious agents and syndromes (CQLR, c. P-42, r. 4.2). Furthermore, it ensures adequate protection against infestation by amitraz-resistant strains of Varroa destructor. This protocol targets beekeeping operations established in a region outside Québec that is not considered to be at risk of infestation by the small beehive beetle (SHB) and specifies the requirements that these businesses must meet to be eligible for an authorization to import into the territory of Québec.